本实验结果还显示:两种菌毛间的氨基酸组成相同,百分含量也大致相同。天门冬氨酸、谷氨酸、丙氨酸、赖氨酸等含量较多。与国外的研究结果比较,两种菌毛与菌株T14V、WVU627菌毛的氨基酸百分组成大部分一致［2～4］。并且都以非极性氨基酸百分含量最高,菌株T14V菌毛中占29%［2］或35%［3］,菌株WVU627菌毛中占38%［4］,本实验提取的菌毛中占38.6%(Ⅰ型)和38.9%(Ⅱ型)。这些结果均提示,菌毛中存在高度疏水性片段。另外,检测到的碱性氨基酸百分含量均最低。
　　作者曾试图通过SDS-PAGE确定菌毛纯度,并离解菌毛,测定菌毛分子量,以及了解菌毛亚单位,但实验结果,两种菌毛蛋白质均未进胶。这与Clark等［2］实验结果一致。因此,需要进一步研究。

本课题为国家自然科学基金资助项目(编号 39270717)

作者单位：李　文　刘天佳　华西医科大学口腔医学院　610041
　　　　　章崇杰　赵崇蓉　基础医学院

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